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KIF22 antibody [N3C2], Internal

KO/KD
Anti-KIF22 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112357
Anti-KIF22 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112357
Anti-KIF22 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112357
Anti-KIF22 antibody [N3C2], Internal used in Western Blot (WB). GTX112357

Cat. No. GTX112357

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all KIF22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

IMR-32 , SK-N-AS

Product Note

Knockdown/Knockout validation was supported by references (PMID:29382784)

Predict Reactivity

Mouse, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KIF22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166142

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 22 , A-328A3.2 , KID , KNSL4 , OBP , OBP-1 , OBP-2 , SEMDJL2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of kinesin-like protein family. This family of proteins are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KIF22 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112357

GTX112357 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using KIF22(GTX112357) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KIF22 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112357

GTX112357 ICC/IF Image

KIF22 antibody [N3C2], Internal detects KIF22 protein at cytoskeleton by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KIF22 protein stained by KIF22 antibody [N3C2], Internal (GTX112357) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-KIF22 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112357

GTX112357 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using KIF22(GTX112357) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KIF22 antibody [N3C2], Internal used in Western Blot (WB). GTX112357

GTX112357 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF22 antibody [N3C2], Internal (GTX112357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Thompson AF et al. Elife 2022; 11 Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation.
Application : ICC/IF
Reactivity : Human
Pike R et al. Sci Signal 2018; 11 (515) KIF22 coordinates CAR and EGFR dynamics to promote cancer cell proliferation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169